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Then cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kits (4374967, Applied Biosystems) with the accompanied random primers, as described previously (Ren et al., 2021)

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The process works through several key steps: Folate converts to 5-methyltetrahydrofolate (5-methylTHF), which circulates in blood This form donates methyl groups necessary for DNA methylation After transferring methyl groups, SAM becomes S-adenosylhomocysteine (SAH) Much of American men over age 60 take dietary supplements containing folic acid [20]